Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Jeeyun Lee; Young Hyuck Im; Hyun Jung Hae; Hyun Kim Joo; Oh Park Joon; Kihyun Kim; Seog Kim Won; Seok Ahn Jin; Won Jung Chul; Suk Park Young; Ki Kang Won; Keunchil Park

doi:10.1016/j.bbrc.2005.06.093

Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Jeeyun Lee, Young Hyuck Im, Hyun Jung Hae, Hyun Kim Joo, Oh Park Joon, Kihyun Kim, Seog Kim Won, Seok Ahn Jin, Won Jung Chul, Suk Park Young, Ki Kang Won, Keunchil Park

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-³²P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

Original language	English
Pages (from-to)	313-318
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	334
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.06.093
State	Published - 26 Aug 2005
Externally published	Yes

Keywords

Curcumin
Cyclooxygenase-2
Interferon-α
NF-κB
Non-small cell lung cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2005.06.093

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@article{dc1ce67ec9594aa9bd0d5ac323e7b681,

title = "Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells",

abstract = "The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.",

keywords = "Curcumin, Cyclooxygenase-2, Interferon-α, NF-κB, Non-small cell lung cancer",

author = "Jeeyun Lee and Im, \{Young Hyuck\} and Hae, \{Hyun Jung\} and Joo, \{Hyun Kim\} and Joon, \{Oh Park\} and Kihyun Kim and Won, \{Seog Kim\} and Jin, \{Seok Ahn\} and Chul, \{Won Jung\} and Young, \{Suk Park\} and Won, \{Ki Kang\} and Keunchil Park",

year = "2005",

month = aug,

day = "26",

doi = "10.1016/j.bbrc.2005.06.093",

language = "English",

volume = "334",

pages = "313--318",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

AU - Lee, Jeeyun

AU - Im, Young Hyuck

AU - Hae, Hyun Jung

AU - Joo, Hyun Kim

AU - Joon, Oh Park

AU - Kim, Kihyun

AU - Won, Seog Kim

AU - Jin, Seok Ahn

AU - Chul, Won Jung

AU - Young, Suk Park

AU - Won, Ki Kang

AU - Park, Keunchil

PY - 2005/8/26

Y1 - 2005/8/26

N2 - The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

AB - The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

KW - Curcumin

KW - Cyclooxygenase-2

KW - Interferon-α

KW - NF-κB

KW - Non-small cell lung cancer

UR - https://www.scopus.com/pages/publications/22144482343

U2 - 10.1016/j.bbrc.2005.06.093

DO - 10.1016/j.bbrc.2005.06.093

M3 - Article

C2 - 16005433

AN - SCOPUS:22144482343

SN - 0006-291X

VL - 334

SP - 313

EP - 318

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Abstract

Keywords

UN SDGs

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