Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Jeeyun Lee; Young Hyuck Im; Hyun Jung Hae; Hyun Kim Joo; Oh Park Joon; Kihyun Kim; Seog Kim Won; Seok Ahn Jin; Won Jung Chul; Suk Park Young; Ki Kang Won; Keunchil Park

doi:10.1016/j.bbrc.2005.06.093

Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Jeeyun Lee
, Young Hyuck Im
, Hyun Jung Hae
, Hyun Kim Joo
, Oh Park Joon
, Kihyun Kim
, Seog Kim Won
, Seok Ahn Jin
, Won Jung Chul
, Suk Park Young
, Ki Kang Won
, Keunchil Park

Department of Internal Medicine

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-³²P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

Original language	English
Pages (from-to)	313-318
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	334
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.06.093
State	Published - 26 Aug 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Curcumin
Cyclooxygenase-2
Interferon-α
NF-κB
Non-small cell lung cancer

Access to Document

10.1016/j.bbrc.2005.06.093

Cite this

@article{dc1ce67ec9594aa9bd0d5ac323e7b681,

title = "Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells",

abstract = "The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.",

keywords = "Curcumin, Cyclooxygenase-2, Interferon-α, NF-κB, Non-small cell lung cancer",

author = "Jeeyun Lee and Im, \{Young Hyuck\} and Hae, \{Hyun Jung\} and Joo, \{Hyun Kim\} and Joon, \{Oh Park\} and Kihyun Kim and Won, \{Seog Kim\} and Jin, \{Seok Ahn\} and Chul, \{Won Jung\} and Young, \{Suk Park\} and Won, \{Ki Kang\} and Keunchil Park",

year = "2005",

month = aug,

day = "26",

doi = "10.1016/j.bbrc.2005.06.093",

language = "English",

volume = "334",

pages = "313--318",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

AU - Lee, Jeeyun

AU - Im, Young Hyuck

AU - Hae, Hyun Jung

AU - Joo, Hyun Kim

AU - Joon, Oh Park

AU - Kim, Kihyun

AU - Won, Seog Kim

AU - Jin, Seok Ahn

AU - Chul, Won Jung

AU - Young, Suk Park

AU - Won, Ki Kang

AU - Park, Keunchil

PY - 2005/8/26

Y1 - 2005/8/26

N2 - The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

AB - The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-α treatment. The IFN-α-treated A549 cells showed increase in protein expression levels of NF-κB and COX-2. IFN-α induced NF-κB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-α-induced COX-2 expression in A549 cells. Within 10 min, IFN-α rapidly induced the binding activity of a γ-32P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-α-induced activations of NF-κB and COX-2 were inhibited by the addition of curcumin in A549 cells.

KW - Curcumin

KW - Cyclooxygenase-2

KW - Interferon-α

KW - NF-κB

KW - Non-small cell lung cancer

UR - https://www.scopus.com/pages/publications/22144482343

U2 - 10.1016/j.bbrc.2005.06.093

DO - 10.1016/j.bbrc.2005.06.093

M3 - Article

C2 - 16005433

AN - SCOPUS:22144482343

SN - 0006-291X

VL - 334

SP - 313

EP - 318

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Curcumin inhibits interferon-α induced NF-κB and COX-2 in human A549 non-small cell lung cancer cells

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this