Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

Eshan Ghosh; Hemlata Dwivedi; Mithu Baidya; Ashish Srivastava; Punita Kumari; Tomek Stepniewski; Hee Ryung Kim; Mi Hye Lee; Jaana van Gastel; Madhu Chaturvedi; Debarati Roy; Shubhi Pandey; Jagannath Maharana; Ramon Guixà-González; Louis M. Luttrell; Ka Young Chung; Somnath Dutta; Jana Selent; Arun K. Shukla

doi:10.1016/j.celrep.2019.08.053

Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

Eshan Ghosh
, Hemlata Dwivedi
, Mithu Baidya
, Ashish Srivastava
, Punita Kumari
, Tomek Stepniewski
, Hee Ryung Kim
, Mi Hye Lee
, Jaana van Gastel
, Madhu Chaturvedi
, Debarati Roy
, Shubhi Pandey
, Jagannath Maharana
, Ramon Guixà-González
, Louis M. Luttrell
, Ka Young Chung
, Somnath Dutta
, Jana Selent
, Arun K. Shukla

Department of Pharmacy

Indian Institute of Technology Kanpur
Pompeu Fabra University
Sungkyunkwan University
Medical University of South Carolina
Flanders Institute for Biotechnology
University of Antwerp
Autonomous University of Barcelona
Department of Veterans Affairs
Indian Institute of Science Bangalore

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

Original language	English
Pages (from-to)	3287-3299.e6
Journal	Cell Reports
Volume	28
Issue number	13
DOIs	https://doi.org/10.1016/j.celrep.2019.08.053
State	Published - 24 Sep 2019

Keywords

GPCRs
antibody fragments
biased agonism
biosensors
cellular signaling
desensitization
electron microscopy
negative staining
β-arrestins

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10.1016/j.celrep.2019.08.053

Cite this

Ghosh, E., Dwivedi, H., Baidya, M., Srivastava, A., Kumari, P., Stepniewski, T., Kim, H. R., Lee, M. H., van Gastel, J., Chaturvedi, M., Roy, D., Pandey, S., Maharana, J., Guixà-González, R., Luttrell, L. M., Chung, K. Y., Dutta, S., Selent, J., & Shukla, A. K. (2019). Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms. Cell Reports, 28(13), 3287-3299.e6. https://doi.org/10.1016/j.celrep.2019.08.053

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title = "Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms",

abstract = "Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.",

keywords = "GPCRs, antibody fragments, biased agonism, biosensors, cellular signaling, desensitization, electron microscopy, negative staining, β-arrestins",

author = "Eshan Ghosh and Hemlata Dwivedi and Mithu Baidya and Ashish Srivastava and Punita Kumari and Tomek Stepniewski and Kim, \{Hee Ryung\} and Lee, \{Mi Hye\} and \{van Gastel\}, Jaana and Madhu Chaturvedi and Debarati Roy and Shubhi Pandey and Jagannath Maharana and Ramon Guix{\`a}-Gonz{\'a}lez and Luttrell, \{Louis M.\} and Chung, \{Ka Young\} and Somnath Dutta and Jana Selent and Shukla, \{Arun K.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = sep,

day = "24",

doi = "10.1016/j.celrep.2019.08.053",

language = "English",

volume = "28",

pages = "3287--3299.e6",

journal = "Cell Reports",

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Ghosh, E, Dwivedi, H, Baidya, M, Srivastava, A, Kumari, P, Stepniewski, T, Kim, HR, Lee, MH, van Gastel, J, Chaturvedi, M, Roy, D, Pandey, S, Maharana, J, Guixà-González, R, Luttrell, LM, Chung, KY, Dutta, S, Selent, J & Shukla, AK 2019, 'Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms', Cell Reports, vol. 28, no. 13, pp. 3287-3299.e6. https://doi.org/10.1016/j.celrep.2019.08.053

TY - JOUR

T1 - Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

AU - Ghosh, Eshan

AU - Dwivedi, Hemlata

AU - Baidya, Mithu

AU - Srivastava, Ashish

AU - Kumari, Punita

AU - Stepniewski, Tomek

AU - Kim, Hee Ryung

AU - Lee, Mi Hye

AU - van Gastel, Jaana

AU - Chaturvedi, Madhu

AU - Roy, Debarati

AU - Pandey, Shubhi

AU - Maharana, Jagannath

AU - Guixà-González, Ramon

AU - Luttrell, Louis M.

AU - Chung, Ka Young

AU - Dutta, Somnath

AU - Selent, Jana

AU - Shukla, Arun K.

PY - 2019/9/24

Y1 - 2019/9/24

N2 - Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

AB - Ghosh et al. discover structural differences between β-arrestin isoforms (β-arrestin 1 and 2), which are universal regulators of signaling and trafficking of G-protein-coupled receptors (GPCRs). These findings have direct implications for understanding the regulatory and signaling paradigms of GPCRs and designing novel therapeutics targeting this important class of receptors.

KW - GPCRs

KW - antibody fragments

KW - biased agonism

KW - biosensors

KW - cellular signaling

KW - desensitization

KW - electron microscopy

KW - negative staining

KW - β-arrestins

UR - https://www.scopus.com/pages/publications/85072193756

U2 - 10.1016/j.celrep.2019.08.053

DO - 10.1016/j.celrep.2019.08.053

M3 - Article

C2 - 31553900

AN - SCOPUS:85072193756

SN - 2211-1247

VL - 28

SP - 3287-3299.e6

JO - Cell Reports

JF - Cell Reports

IS - 13

ER -

Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between β-Arrestin Isoforms

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Keywords

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