TY - JOUR
T1 - Confluentibacter lentus gen. Nov., sp. nov., isolated from the junction between the ocean and a freshwater lake
AU - Park, Sooyeon
AU - Kim, Sona
AU - Jung, Yong Taek
AU - Park, Jin Min
AU - Yoon, Jung Hoon
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2016/2
Y1 - 2016/2
N2 - A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-3T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo on the East Sea, South Korea, and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30–35 °C, at pH 7.0–7.5 and in the presence of 1.0–2.0% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HJM-3T clustered with the type strain of Yeosuana aromativorans and the proposed type strain of Mariniflexile ostreae. Strain HJM-3T exhibited the highest 16S rRNA gene sequence similarity values (each 95.1 %) to these representative strains of Y. aromativorans and M. ostreae, and sequence similarities of less than 94.9% to the type strains of other recognized species. Strain HJM-3T contained menaquinone-6 as the predominant isoprenoid quinone, and iso-C15: 0, iso-C15: 1 G and iso-C17: 0 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-3T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-3T was 34.7 mol%. The fatty acid and polar lipid profiles and DNA G+C content of strain HJM-3T could be distinguished from those of the phylogenetically related taxa. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain HJM-3T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Confluentibacter lentus gen. nov., sp. nov., is proposed. The type strain is HJM-3T (=KCTC 42777T=NBRC 111588T).
AB - A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-3T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo on the East Sea, South Korea, and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30–35 °C, at pH 7.0–7.5 and in the presence of 1.0–2.0% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HJM-3T clustered with the type strain of Yeosuana aromativorans and the proposed type strain of Mariniflexile ostreae. Strain HJM-3T exhibited the highest 16S rRNA gene sequence similarity values (each 95.1 %) to these representative strains of Y. aromativorans and M. ostreae, and sequence similarities of less than 94.9% to the type strains of other recognized species. Strain HJM-3T contained menaquinone-6 as the predominant isoprenoid quinone, and iso-C15: 0, iso-C15: 1 G and iso-C17: 0 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-3T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-3T was 34.7 mol%. The fatty acid and polar lipid profiles and DNA G+C content of strain HJM-3T could be distinguished from those of the phylogenetically related taxa. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain HJM-3T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Confluentibacter lentus gen. nov., sp. nov., is proposed. The type strain is HJM-3T (=KCTC 42777T=NBRC 111588T).
UR - https://www.scopus.com/pages/publications/84960465584
U2 - 10.1099/ijsem.0.000804
DO - 10.1099/ijsem.0.000804
M3 - Article
AN - SCOPUS:84960465584
SN - 1466-5026
VL - 66
SP - 868
EP - 873
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 2
M1 - 000804
ER -