TY - JOUR
T1 - Complete genome sequence of acetate-producing Klebsiella pneumoniae L5-2 isolated from infant feces
AU - Park, Yong Soo
AU - Kang, Jisu
AU - Chung, Won Hyong
AU - Lim, Mi Young
AU - Seo, Myung Ji
AU - Nam, Young Do
AU - Yoon, Jung Hoon
AU - Seo, Dong Ho
N1 - Publisher Copyright:
© 2019, King Abdulaziz City for Science and Technology.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Acetate is an important metabolite in infants as it can affect metabolism as well as immune and inflammatory responses. However, there have been no studies on acetate production by Klebsiella pneumoniae isolated from infant feces. In this study, we isolated a K. pneumoniae strain, L5-2, from infant feces, and we found it produces acetate. The genome of L5-2 consisted of a 5,237,123-bp single chromosome and a 139,211-bp single plasmid. The G + C content was 57.27%. By whole-genome analysis of K. pneumoniae L5-2, we identified seven genes related to acetate production (poxA, pta, eutD, ackA, eutP, eutQ, and adhE). We confirmed acetate production by K. pneumoniae L5-2 by ion chromatography. The aldehyde/alcohol dehydrogenase (adhE) activity of K. pneumoniae L5-2 was significantly higher than that of the K. pneumoniae subsp. ozaenae ATCC 11296. Thus, the acetate-producing ability of K. pneumoniae L5-2 was influenced by the adhE gene. In addition, K. pneumoniae L5-2 had significantly less virulence factor-encoding genes than other K. pneumoniae strains isolated from humans. In conclusion, K. pneumoniae L5-2 isolated from infant feces has less virulence factors and higher adhE activity than other K. pneumoniae strains.
AB - Acetate is an important metabolite in infants as it can affect metabolism as well as immune and inflammatory responses. However, there have been no studies on acetate production by Klebsiella pneumoniae isolated from infant feces. In this study, we isolated a K. pneumoniae strain, L5-2, from infant feces, and we found it produces acetate. The genome of L5-2 consisted of a 5,237,123-bp single chromosome and a 139,211-bp single plasmid. The G + C content was 57.27%. By whole-genome analysis of K. pneumoniae L5-2, we identified seven genes related to acetate production (poxA, pta, eutD, ackA, eutP, eutQ, and adhE). We confirmed acetate production by K. pneumoniae L5-2 by ion chromatography. The aldehyde/alcohol dehydrogenase (adhE) activity of K. pneumoniae L5-2 was significantly higher than that of the K. pneumoniae subsp. ozaenae ATCC 11296. Thus, the acetate-producing ability of K. pneumoniae L5-2 was influenced by the adhE gene. In addition, K. pneumoniae L5-2 had significantly less virulence factor-encoding genes than other K. pneumoniae strains isolated from humans. In conclusion, K. pneumoniae L5-2 isolated from infant feces has less virulence factors and higher adhE activity than other K. pneumoniae strains.
KW - Acetate
KW - Gut microbiota
KW - Infant
KW - Klebsiella pneumoniae
KW - Short chain fatty acid
KW - Whole-genome sequencing
UR - https://www.scopus.com/pages/publications/85061638509
U2 - 10.1007/s13205-019-1578-y
DO - 10.1007/s13205-019-1578-y
M3 - Article
AN - SCOPUS:85061638509
SN - 2190-572X
VL - 9
JO - 3 Biotech
JF - 3 Biotech
IS - 3
M1 - 84
ER -
