Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines

Soo Jae Lee; Kyun Hwan Kim; Ji Sook Park; Jin Woo Jung; Young Hwan Kim; Sang Kyung Kim; Wan Seok Kim; Hyun gyung Goh; Soo hyun Kim; Jung Sun Yoo; Dong Wook Kim; Kwang Pyo Kim

doi:10.1016/j.bbrc.2007.03.191

Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines

Soo Jae Lee
, Kyun Hwan Kim
, Ji Sook Park
, Jin Woo Jung
, Young Hwan Kim
, Sang Kyung Kim
, Wan Seok Kim
, Hyun gyung Goh
, Soo hyun Kim
, Jung Sun Yoo
, Dong Wook Kim
, Kwang Pyo Kim

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.

Original language	English
Pages (from-to)	620-626
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.03.191
State	Published - 8 Jun 2007
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acute promyelocytic leukemia
Chronic myeloid leukemia
Mass spectrometry
Proteomics

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10.1016/j.bbrc.2007.03.191

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title = "Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines",

abstract = "This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.",

keywords = "Acute promyelocytic leukemia, Chronic myeloid leukemia, Mass spectrometry, Proteomics",

author = "Lee, \{Soo Jae\} and Kim, \{Kyun Hwan\} and Park, \{Ji Sook\} and Jung, \{Jin Woo\} and Kim, \{Young Hwan\} and Kim, \{Sang Kyung\} and Kim, \{Wan Seok\} and Goh, \{Hyun gyung\} and Kim, \{Soo hyun\} and Yoo, \{Jung Sun\} and Kim, \{Dong Wook\} and Kim, \{Kwang Pyo\}",

year = "2007",

month = jun,

day = "8",

doi = "10.1016/j.bbrc.2007.03.191",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines

AU - Lee, Soo Jae

AU - Kim, Kyun Hwan

AU - Park, Ji Sook

AU - Jung, Jin Woo

AU - Kim, Young Hwan

AU - Kim, Sang Kyung

AU - Kim, Wan Seok

AU - Goh, Hyun gyung

AU - Kim, Soo hyun

AU - Yoo, Jung Sun

AU - Kim, Dong Wook

AU - Kim, Kwang Pyo

PY - 2007/6/8

Y1 - 2007/6/8

N2 - This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.

AB - This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.

KW - Acute promyelocytic leukemia

KW - Chronic myeloid leukemia

KW - Mass spectrometry

KW - Proteomics

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DO - 10.1016/j.bbrc.2007.03.191

M3 - Article

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AN - SCOPUS:34247478903

SN - 0006-291X

VL - 357

SP - 620

EP - 626

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines

Abstract

UN SDGs

Keywords

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