Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion

Woo Kyun Oh; Jae Cheal Yoo; Donggyu Jo; Young Hwa Song; Moon Gyo Kim; Dongeun Park

doi:10.1006/bbrc.1997.6875

Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion

Woo Kyun Oh
, Jae Cheal Yoo
, Donggyu Jo
, Young Hwa Song
, Moon Gyo Kim
, Dongeun Park

Gwangju Institute of Science and Technology
National Institutes of Health

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

A mouse thymus cDNA expression library was screened with monoclonal antibody (mAb), B16-5 which binds to common epitope in SH3 domains of phospholipase C-γ 1 (PLC-γ 1) and Nck. We have determined the complete nucleotide sequence of one of several positive clones. The 4,172 bp cDNA clone (Gen-Bank Accession No. U96634) encodes a SH3 domain-containing protein of 646 amino acids. Besides the SH3 domain, the predicted protein has a proline-rich region, nuclear localization signals, and leucine zipper motifs. The expressed protein in Sf9 insect cell exhibits a polypeptide of 85 kDa on SDS-PAGE. The protein is widely distributed in rat tissue with an especially high level of expression in brain and testis. Interestingly, the specific antibodies detected four related proteins of different size (75, 85, 90 and 105 kDa) in brain. In A431 cell, p85SPR is enriched at focal adhesion points indicating that the protein may interact with protein(s) in focal complexes.

Original language	English
Pages (from-to)	794-798
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	235
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1997.6875
State	Published - 27 Jun 1997
Externally published	Yes

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title = "Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion",

abstract = "A mouse thymus cDNA expression library was screened with monoclonal antibody (mAb), B16-5 which binds to common epitope in SH3 domains of phospholipase C-γ 1 (PLC-γ 1) and Nck. We have determined the complete nucleotide sequence of one of several positive clones. The 4,172 bp cDNA clone (Gen-Bank Accession No. U96634) encodes a SH3 domain-containing protein of 646 amino acids. Besides the SH3 domain, the predicted protein has a proline-rich region, nuclear localization signals, and leucine zipper motifs. The expressed protein in Sf9 insect cell exhibits a polypeptide of 85 kDa on SDS-PAGE. The protein is widely distributed in rat tissue with an especially high level of expression in brain and testis. Interestingly, the specific antibodies detected four related proteins of different size (75, 85, 90 and 105 kDa) in brain. In A431 cell, p85SPR is enriched at focal adhesion points indicating that the protein may interact with protein(s) in focal complexes.",

author = "Oh, \{Woo Kyun\} and Yoo, \{Jae Cheal\} and Donggyu Jo and Song, \{Young Hwa\} and Kim, \{Moon Gyo\} and Dongeun Park",

year = "1997",

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doi = "10.1006/bbrc.1997.6875",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion

AU - Oh, Woo Kyun

AU - Yoo, Jae Cheal

AU - Jo, Donggyu

AU - Song, Young Hwa

AU - Kim, Moon Gyo

AU - Park, Dongeun

PY - 1997/6/27

Y1 - 1997/6/27

N2 - A mouse thymus cDNA expression library was screened with monoclonal antibody (mAb), B16-5 which binds to common epitope in SH3 domains of phospholipase C-γ 1 (PLC-γ 1) and Nck. We have determined the complete nucleotide sequence of one of several positive clones. The 4,172 bp cDNA clone (Gen-Bank Accession No. U96634) encodes a SH3 domain-containing protein of 646 amino acids. Besides the SH3 domain, the predicted protein has a proline-rich region, nuclear localization signals, and leucine zipper motifs. The expressed protein in Sf9 insect cell exhibits a polypeptide of 85 kDa on SDS-PAGE. The protein is widely distributed in rat tissue with an especially high level of expression in brain and testis. Interestingly, the specific antibodies detected four related proteins of different size (75, 85, 90 and 105 kDa) in brain. In A431 cell, p85SPR is enriched at focal adhesion points indicating that the protein may interact with protein(s) in focal complexes.

AB - A mouse thymus cDNA expression library was screened with monoclonal antibody (mAb), B16-5 which binds to common epitope in SH3 domains of phospholipase C-γ 1 (PLC-γ 1) and Nck. We have determined the complete nucleotide sequence of one of several positive clones. The 4,172 bp cDNA clone (Gen-Bank Accession No. U96634) encodes a SH3 domain-containing protein of 646 amino acids. Besides the SH3 domain, the predicted protein has a proline-rich region, nuclear localization signals, and leucine zipper motifs. The expressed protein in Sf9 insect cell exhibits a polypeptide of 85 kDa on SDS-PAGE. The protein is widely distributed in rat tissue with an especially high level of expression in brain and testis. Interestingly, the specific antibodies detected four related proteins of different size (75, 85, 90 and 105 kDa) in brain. In A431 cell, p85SPR is enriched at focal adhesion points indicating that the protein may interact with protein(s) in focal complexes.

UR - https://www.scopus.com/pages/publications/0031587921

U2 - 10.1006/bbrc.1997.6875

DO - 10.1006/bbrc.1997.6875

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AN - SCOPUS:0031587921

SN - 0006-291X

VL - 235

SP - 794

EP - 798

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion

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