Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A

Kyeong Kyu Kim; Hisao Yokota; Rosalind Kim; Sung Hou Kim

doi:10.1002/pro.5560061023

Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A

Kyeong Kyu Kim, Hisao Yokota, Rosalind Kim, Sung Hou Kim

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4₁22 with unit cell dimensions a = b = 45.52 Å and c = 155.59 Å. These crystals diffract to at least 2.2 Å resolution.

Original language	English
Pages (from-to)	2268-2270
Number of pages	3
Journal	Protein Science
Volume	6
Issue number	10
DOIs	https://doi.org/10.1002/pro.5560061023
State	Published - Oct 1997
Externally published	Yes

Keywords

Hyperthermophile
Methanococcus jannaschii
Translation initiation factor
X-ray crystallography

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10.1002/pro.5560061023

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title = "Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A",

abstract = "A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8\% PEG 4000 as precipitant and belong to space group P4122 with unit cell dimensions a = b = 45.52 {\AA} and c = 155.59 {\AA}. These crystals diffract to at least 2.2 {\AA} resolution.",

keywords = "Hyperthermophile, Methanococcus jannaschii, Translation initiation factor, X-ray crystallography",

author = "Kim, \{Kyeong Kyu\} and Hisao Yokota and Rosalind Kim and Kim, \{Sung Hou\}",

year = "1997",

month = oct,

doi = "10.1002/pro.5560061023",

language = "English",

volume = "6",

pages = "2268--2270",

journal = "Protein Science",

issn = "0961-8368",

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T1 - Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A

AU - Kim, Kyeong Kyu

AU - Yokota, Hisao

AU - Kim, Rosalind

AU - Kim, Sung Hou

PY - 1997/10

Y1 - 1997/10

N2 - A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4122 with unit cell dimensions a = b = 45.52 Å and c = 155.59 Å. These crystals diffract to at least 2.2 Å resolution.

AB - A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4122 with unit cell dimensions a = b = 45.52 Å and c = 155.59 Å. These crystals diffract to at least 2.2 Å resolution.

KW - Hyperthermophile

KW - Methanococcus jannaschii

KW - Translation initiation factor

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/0030817185

U2 - 10.1002/pro.5560061023

DO - 10.1002/pro.5560061023

M3 - Article

C2 - 9336851

AN - SCOPUS:0030817185

SN - 0961-8368

VL - 6

SP - 2268

EP - 2270

JO - Protein Science

JF - Protein Science

IS - 10

ER -

Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A

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Keywords

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