TY - JOUR
T1 - Citrobacter bitternis sp. nov. Isolated from Bitterns
AU - Ko, Kwan Soo
AU - Choi, Ji Young
AU - Kim, Joo
AU - Park, Myoung Kyu
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - In this study, we reported two gram-negative bacteria that were isolated from bitterns, designated as SKKU-TP7T and SKKU-TP20, representing a novel species of Citrobacter. Based on the 16S rRNA gene sequences, the two strains were found to be closely related and showed the highest pairwise similarity with Citrobacter farmeri CDC 2992-81T (97.1–97.3 %) and other Citrobacter species. Cellular fatty acid analysis revealed that the profiles of strains SKKU-TP7T and SKKU-TP20 were similar to those of related species of Citrobacter. The major cellular fatty acids were C16:0 (31.5 %), summed feature 3 (C16:1 ω7c, C16:1 ω6c, 19.7 %), summed feature 8 (C18:1 ω7c, C18:1 ω6c, 11.9 %), C17:0 cyclo (10.7 %), and summed feature 2 (C12:0 aldehyde/unknown 10928, 9.5 %). Although the strains could utilize sucrose and raffinose as a carbon source, they did not produce ornithine decarboxylase and urease. The biochemical and genotypic characteristics indicate that strains SKKU-TP7T and SKKU-TP20 represent a novel species of Citrobacter, for which the name Citrobacter bitterns sp. nov. is proposed. The type strain is SKKU-TP7T (=KCTC 42139T = JCM 30009T).
AB - In this study, we reported two gram-negative bacteria that were isolated from bitterns, designated as SKKU-TP7T and SKKU-TP20, representing a novel species of Citrobacter. Based on the 16S rRNA gene sequences, the two strains were found to be closely related and showed the highest pairwise similarity with Citrobacter farmeri CDC 2992-81T (97.1–97.3 %) and other Citrobacter species. Cellular fatty acid analysis revealed that the profiles of strains SKKU-TP7T and SKKU-TP20 were similar to those of related species of Citrobacter. The major cellular fatty acids were C16:0 (31.5 %), summed feature 3 (C16:1 ω7c, C16:1 ω6c, 19.7 %), summed feature 8 (C18:1 ω7c, C18:1 ω6c, 11.9 %), C17:0 cyclo (10.7 %), and summed feature 2 (C12:0 aldehyde/unknown 10928, 9.5 %). Although the strains could utilize sucrose and raffinose as a carbon source, they did not produce ornithine decarboxylase and urease. The biochemical and genotypic characteristics indicate that strains SKKU-TP7T and SKKU-TP20 represent a novel species of Citrobacter, for which the name Citrobacter bitterns sp. nov. is proposed. The type strain is SKKU-TP7T (=KCTC 42139T = JCM 30009T).
UR - https://www.scopus.com/pages/publications/84937757906
U2 - 10.1007/s00284-015-0810-1
DO - 10.1007/s00284-015-0810-1
M3 - Article
C2 - 25833091
AN - SCOPUS:84937757906
SN - 0343-8651
VL - 70
SP - 894
EP - 897
JO - Current Microbiology
JF - Current Microbiology
IS - 6
ER -