Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106^T, was isolated from wastewater collected from a textile dye works in Korea. Strain K106^T grew optimally at pH 7.0-7.5 and 30-37 °C in the presence of 0-1.0% (w/v) NaCl. A, phylogenetic tree based on 16S rRNA gene sequences showed that strain K106^T joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9%. The predominant ubiquinone of strain K106^T was Q-10. The fatty acid profile of strain K106^T was similar to that of C. asaccharovorans DSM 6462^T. Major polar lipids of strain K106^T and C. asaccharovorans DSM 6462^T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G + C content was 68.3 mol%. Strain K106^T exhibited 16S rRNA gene sequence similarity of 96.6 % to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106^T can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106^T represents a novel species of the genus Chelatococcus, for which the name Chelatococcus plaeguensis sp. nov. is proposed. The type strain. is K106^T (= KCTC 12979^T =CCUG 54519^T).