Abstract
A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 1422-1427 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 20 |
| Issue number | 7 |
| DOIs | |
| State | Published - 15 Jan 2005 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Glutamic acid decarboxylase
- Immunosensor
- Protein adsorption
- Self-assembled monolayer
- Streptavidin
- Surface plasmon resonance
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