Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir

A Gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, CH15-1^T, was isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the lateblooming period of cyanobacteria. Strain CH15-1^T grew optimally at pH 7.0 and 30 6C. A phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain CH15-1^T belongs to the genus Arenimonas with the similarity range from 92.6-97.4% and is closely related to Arenimonas oryziterrae YC6267^T (97.4%), Arenimonas composti TR7-09^T (95.4%), Arenimonas metalli CF5-1^T (94.7%), Arenimonas malthae CC-JY-1^T (94.6%) and Arenimonas donghaensis HO3-R19^T (92.6%). However, the DNA-DNA hybridization between strain CH15-1^T and the closest strain, Arenimonas oryziterrae YC6267^T, was 8.9-12.9%. The DNA G+C content was 63.9 mol% compared to A. oryziterrae YC626^T, 65.8 mol%. Strain CH15-1^T included Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. The major fatty acids (.5%) were iso-C_{15: 0}, iso-C_{16: 0}, iso-C_{14: 0}, iso-C_{11: 0} 3-OH, iso-C_{17: 0} and summed feature 9 (iso-C_{17: 1}ω9c and/or C_{16: 0} 10-methyl). On the basis of phylogenetic, phenotypic and genetic data, strain CH15-1^T was classified in the genus Arenimonas as a member of a novel species, for which the name Arenimonas daechungensis sp. nov. is proposed. The type strain is CH15-1^T (5KCTC 23553^T5DSM 24763^T).