Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway

See Hyoung Park; Jae Youl Cho; Sae Woong Oh; Mingyeong Kang; Seung Eun Lee; Ju Ah Yoo; Kwangseon Jung; Jienny Lee; Sang Yeol Lee; Jongsung Lee

doi:10.1016/j.cbi.2018.01.005

Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway

See Hyoung Park
, Jae Youl Cho
, Sae Woong Oh
, Mingyeong Kang
, Seung Eun Lee
, Ju Ah Yoo
, Kwangseon Jung
, Jienny Lee
, Sang Yeol Lee
, Jongsung Lee

Department of Integrative Biotechnology

Hongik University
Sungkyunkwan University
Skincure Life Science Institute
Animal and Plant Quarantine Agency
Gachon University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1α was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE₂ production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-κB. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE₂-cAMP signaling-dependent upregulation of HIF-1α. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	Chemico-Biological Interactions
Volume	282
DOIs	https://doi.org/10.1016/j.cbi.2018.01.005
State	Published - 25 Feb 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Arctigenin
HIF
Human mesenchymal stem cells
Stemness
UVA damage

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10.1016/j.cbi.2018.01.005

Cite this

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title = "Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway",

abstract = "The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1α was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE2 production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-κB. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE2-cAMP signaling-dependent upregulation of HIF-1α. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.",

keywords = "Arctigenin, HIF, Human mesenchymal stem cells, Stemness, UVA damage",

author = "Park, \{See Hyoung\} and Cho, \{Jae Youl\} and Oh, \{Sae Woong\} and Mingyeong Kang and Lee, \{Seung Eun\} and Yoo, \{Ju Ah\} and Kwangseon Jung and Jienny Lee and Lee, \{Sang Yeol\} and Jongsung Lee",

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year = "2018",

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day = "25",

doi = "10.1016/j.cbi.2018.01.005",

language = "English",

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T1 - Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway

AU - Park, See Hyoung

AU - Cho, Jae Youl

AU - Oh, Sae Woong

AU - Kang, Mingyeong

AU - Lee, Seung Eun

AU - Yoo, Ju Ah

AU - Jung, Kwangseon

AU - Lee, Jienny

AU - Lee, Sang Yeol

AU - Lee, Jongsung

PY - 2018/2/25

Y1 - 2018/2/25

N2 - The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1α was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE2 production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-κB. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE2-cAMP signaling-dependent upregulation of HIF-1α. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.

AB - The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1α was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE2 production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-κB. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE2-cAMP signaling-dependent upregulation of HIF-1α. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.

KW - Arctigenin

KW - HIF

KW - Human mesenchymal stem cells

KW - Stemness

KW - UVA damage

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SN - 0009-2797

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JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

ER -

Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway

Abstract

UN SDGs

Keywords

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