Antioxidant and cytoprotective effects of (−)-epigallocatechin-3-(3”-o-methyl) gallate

Reactive oxygen species (ROS) are generated from diverse cellular processes or external sources such as chemicals, pollutants, or ultraviolet (UV) irradiation. Accumulation of radicals causes cell damage that can result in degenerative diseases. Antioxidants remove radicals by eliminating unpaired electrons from other molecules. In skin health, antioxidants are essential to protect cells from the environment and prevent skin aging. (−)-Epigallocatechin-3-(3”-O-methyl) gallate (3”Me-EGCG) has been found in limited oolong teas or green teas with distinctive methylated form, but its precise activities have not been fully elucidated. In this study, we examined the antioxidant roles of 3”Me-EGCG in keratinocytes (HaCaT cells). 3”Me-EGCG showed scavenging effects in cell and cell-free systems. Under H₂O₂ exposure, 3”Me-EGCG recovered cell viability and increased the expression of heme oxygenase 1 (HO-1). Under ultraviolet B (UVB) and sodium nitroprusside (SNP) exposure, 3”Me-EGCG protected keratinocytes and regulated the survival protein AKT1. By regulating the AKT1/NF-κB pathway, 3”Me-EGCG augmented cell survival and proliferation in HaCaT cells. These results indicate that 3”Me-EGCG exhibits antioxidant properties, resulting in cytoprotection against various external stimuli. In conclusion, our findings suggest that 3”Me-EGCG can be used as an ingredient of cosmetic products or health supplements.