Analysis of Novel Antioxidant Sesquarterpenes (C35 Terpenes) Produced in Recombinant Corynebacterium glutamicum

Sambandam Ravikumar; Han Min Woo; Jong il Choi

doi:10.1007/s12010-018-2756-9

Analysis of Novel Antioxidant Sesquarterpenes (C₃₅ Terpenes) Produced in Recombinant Corynebacterium glutamicum

Sambandam Ravikumar
, Han Min Woo
, Jong il Choi

Department of Food Science and Biotechnology

Chonnam National University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtN_aN_cM derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C₃₅ carotenoids. For the first time, novel C₃₅ carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtN_aN_cM genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtN_aN_cM genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtN_aN_cM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtN_cM and pCES-H36-CrtN_aN_c, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.

Original language	English
Pages (from-to)	525-534
Number of pages	10
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	186
Issue number	3
DOIs	https://doi.org/10.1007/s12010-018-2756-9
State	Published - 1 Nov 2018

Keywords

Antioxidant activity
C carotenoids
Corynebacterium glutamicum
CrtMN

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title = "Analysis of Novel Antioxidant Sesquarterpenes (C35 Terpenes) Produced in Recombinant Corynebacterium glutamicum",

abstract = "Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtNaNcM derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C35 carotenoids. For the first time, novel C35 carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtNaNcM genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtNaNcM genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtNaNcM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtNcM and pCES-H36-CrtNaNc, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.",

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author = "Sambandam Ravikumar and Woo, \{Han Min\} and Choi, \{Jong il\}",

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AU - Choi, Jong il

PY - 2018/11/1

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N2 - Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtNaNcM derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C35 carotenoids. For the first time, novel C35 carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtNaNcM genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtNaNcM genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtNaNcM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtNcM and pCES-H36-CrtNaNc, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.

AB - Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtNaNcM derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C35 carotenoids. For the first time, novel C35 carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtNaNcM genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtNaNcM genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtNaNcM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtNcM and pCES-H36-CrtNaNc, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.

KW - Antioxidant activity

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KW - Corynebacterium glutamicum

KW - CrtMN

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JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

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ER -

Analysis of Novel Antioxidant Sesquarterpenes (C₃₅ Terpenes) Produced in Recombinant Corynebacterium glutamicum

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