Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)

Young Mi Lee; Il Chan Kim; Sang Oun Jung; Jae Seong Lee

doi:10.1016/j.marpolbul.2005.02.014

Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)

Young Mi Lee
, Il Chan Kim
, Sang Oun Jung
, Jae Seong Lee

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using λZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.

Original language	English
Pages (from-to)	757-768
Number of pages	12
Journal	Marine Pollution Bulletin
Volume	51
Issue number	8-12
DOIs	https://doi.org/10.1016/j.marpolbul.2005.02.014
State	Published - 2005
Externally published	Yes

Keywords

Copepod
EST
Expressed sequence tag
Tigriopus japonicus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.marpolbul.2005.02.014

Cite this

@article{4cf9e966c8774a07addc6d319c124803,

title = "Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)",

abstract = "The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using λZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.",

keywords = "Copepod, EST, Expressed sequence tag, Tigriopus japonicus",

author = "Lee, \{Young Mi\} and Kim, \{Il Chan\} and Jung, \{Sang Oun\} and Lee, \{Jae Seong\}",

year = "2005",

doi = "10.1016/j.marpolbul.2005.02.014",

language = "English",

volume = "51",

pages = "757--768",

journal = "Marine Pollution Bulletin",

issn = "0025-326X",

publisher = "Elsevier Ltd",

number = "8-12",

}

TY - JOUR

T1 - Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)

AU - Lee, Young Mi

AU - Kim, Il Chan

AU - Jung, Sang Oun

AU - Lee, Jae Seong

PY - 2005

Y1 - 2005

N2 - The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using λZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.

AB - The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using λZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.

KW - Copepod

KW - EST

KW - Expressed sequence tag

KW - Tigriopus japonicus

UR - https://www.scopus.com/pages/publications/27744488989

U2 - 10.1016/j.marpolbul.2005.02.014

DO - 10.1016/j.marpolbul.2005.02.014

M3 - Article

C2 - 16291190

AN - SCOPUS:27744488989

SN - 0025-326X

VL - 51

SP - 757

EP - 768

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 8-12

ER -

Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this