An HPLC-UV-based quantitative analytical method for Chrysanthemum morifolium: Development, validation, and application

A simple and reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the analysis of the flowers of Chrysanthemum morifolium (CM). Luteolin-7-Oglucoside (LU7G) was chosen as a target analyte considering its content, availability, and ease of analysis. Chromatographic separation of LU7G was achieved using a Phenomenex Gemini C18 column (250 × 4.6 mm, 5 µm) run with a mobile phase consisting of 0.5% acetic acid in water and 0.5% acetic acid in acetonitrile at a flow rate of 1.0 mL min^-1. The detection wavelength and column temperature were set at 350 nm and 40 oC, respectively. Method validation was performed according to the AOAC guidelines and the method was specific, linear (R² = 0.9991 for 50-300 µg mL^-1), precise (≤ 3.91% RSD), and accurate (100.1-105.7%). The limits of detection and quantification were 3.62 and 10.96 µg mL^-1, respectively. The established method was successfully applied to determine the contents of LU7G in various batches of bulk CM extracts and labscale CM extract. The developed method is a readily applicable method for the quality assessment of CM and its related products.