Abstract
Aim: To investigate whether differential expression of Yes-associated protein (YAP) and β-catenin is important in gastric carcinogenesis. Materials and Methods: A total of 284 paraffin-embedded samples collected from 232 patients with gastric adenocarcinoma were used to evaluate YAP and β-catenin expression by immunohistochemistry, and the experimental findings were compared against those for gastric adenocarcinoma cell lines. Results: Nuclear YAP expression gradually increased from non-neoplastic epithelia to tubular or papillary adenocarcinomas (TPADs) and decreased in signet-ring cell carcinoma (SRCC). Cytoplasmic β-catenin expression increased from non-neoplastic epithelia to highgrade dysplasia and was decreased in TPAD and SRCC. YAP-overexpressing cell lines exhibited marked tumor cell invasion, whereas YAP-depleted cells showed reduced invasion. Conclusion: Nuclear YAP and cytoplasmic β-catenin play important roles in carcinogenesis, and the differential patterns YAP and β-catenin expression between TPAD and SRCC imply the existence of different carcinogenic pathways in these conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 3925-3932 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 35 |
| Issue number | 7 |
| State | Published - 1 Jul 2015 |
| Externally published | Yes |
Keywords
- Adenocarcinoma
- Carcinogenesis
- Gastric neoplasia
- Signet-ring cell carcinoma
- Yes-associated protein
- β-catenin
