Algoriphagus litorisediminis sp. nov., isolated from a tidal flat

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-19^T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-19^T grew optimally at 30°C, at pH 7.0– 8.0 and in the presence of 2.0% (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-19^T belonged to the genus Algoriphagus, clustering with the type strain of Algoriphagus namhaensis, with which it shared 96.5% sequence similarity. Strain OITF-19^T exhibited the highest 16S rRNA gene sequence similarity to Algoriphagus chungangensis CAU 1002^T (97.0%) and of 92.0–96.8% to the type strains of other Algoriphagus species. Strain OITF-19^T contained MK-7 as the predominant menaquinone and summed feature 3 (C_{16: 1}w7c and/or C_{16: 1}w6c), iso-C_{15: 0} and iso-C_{17: 0} 3-OH as the major fatty acids. The major polar lipids detected in strain OITF-19^T were phosphatidylcholine, phosphatidylethanolamine and three unidentified lipids. The DNA G+C content of strain OITF- 19^T was 38.3 mol%. Mean DNA–DNA relatedness between strain OITF-19^T and the type strain of A. chungangensis was 26%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-19^T is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain OITF-19^T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus litorisediminis sp. nov. is proposed. The type strain is OITF-19^T (=KCTC 52456^T=NBRC 112418^T).