Algoriphagus aquaemixtae sp. nov., isolated from water in an estuary environment

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Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped or ovoid bacterium, designated strain KEM-10T, was isolated from water in an estuary environment in the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-10T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KEM-10T belonged to the genus Algoriphagus, joining the type strain of Algoriphagus litorisediminis showing 97.4% sequence similarity. Strain KEM-10T exhibited 16S rRNA gene sequence similarity of 92.2-96.1% to the type strains of the other Algoriphagus species. Strain KEM-10T contained MK-7 as the predominant menaquinone and iso-C15:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c) as the major fatty acids. The major polar lipids detected in strain KEM-10T were phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain KEM-10T was 40.6 mol%. The mean DNA-DNA relatedness value between strain KEM-10T and the type strain of A. litorisediminis was 12%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain KEM-10T is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain KEM-10T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus aquaemixtae sp. nov. is proposed. The type strain is KEM-10T (=KCTC 52839T=NBRC 112780T).

Original languageEnglish
Article number002091
Pages (from-to)3231-3236
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number9
DOIs
StatePublished - Sep 2017

Keywords

  • Algoriphagus aquaemixtae
  • Estuary environment
  • Novel species
  • Polyphasic taxonomy

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