Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect

Ja Young Seol; Kyung Ho Park; Chang Il Hwang; Woong Yang Park; Chul Gyu Yoo; Young Whan Kim; Sung Koo Han; Young Soo Shim; Choon Taek Lee

doi:10.1038/sj.cgt.7700597

Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect

Ja Young Seol
, Kyung Ho Park
, Chang Il Hwang
, Woong Yang Park
, Chul Gyu Yoo
, Young Whan Kim
, Sung Koo Han
, Young Soo Shim
, Choon Taek Lee

Seoul National University

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

TRAIL is a cytokine with a unique ability to induce apoptosis selectively in many transformed cell lines. The instability of TRAIL and the resistance of some cancer cells to TRAIL present the main obstacles for clinical experimentation. We generated an adenovirus expressing full-length TRAIL and tested its efficacy in several cancer cell lines. Ad-TRAIL-infected cancer cells localized full-length TRAIL protein to the cytoplasm and released same-sized TRAIL in the media. Ad-TRAIL was found to induce apoptotic cell death in several cancer cell lines resistant to soluble TRAIL (A549, SKOV3, HT-29 and LNCap) and in TRAIL-sensitive cell lines. Ad-TRAIL, but not soluble TRAIL, induced apoptotic cell death in TRAIL-resistant cell lines, manifested by an increased sub-G1 proportion, caspase-3 activation and PARP cleavage. Ad-TRAIL also induced a media-transferable bystander effect, but only in soluble TRAIL-sensitive cell lines. In conclusion, two novel characteristics of ad-TRAIL were found during this study. First, that ad-TRAIL can induce apoptotic cell death in several cancer cell lines resistant to sTRAIL. Second, that ad-TRAIL induces a media-transferable bystander effect, which is expected to increase its therapeutic value by allowing TRAIL to overcome the locally acting nature and low transduction rate commonly encountered in clinical situation.

Original language	English
Pages (from-to)	540-548
Number of pages	9
Journal	Cancer Gene Therapy
Volume	10
Issue number	7
DOIs	https://doi.org/10.1038/sj.cgt.7700597
State	Published - 1 Jul 2003
Externally published	Yes

Keywords

Adenovirus
Apoptosis
Bystander effect
TRAIL
TRAIL resistance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.cgt.7700597

Cite this

@article{89b2ae42f07b459792c984249122aeea,

title = "Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect",

abstract = "TRAIL is a cytokine with a unique ability to induce apoptosis selectively in many transformed cell lines. The instability of TRAIL and the resistance of some cancer cells to TRAIL present the main obstacles for clinical experimentation. We generated an adenovirus expressing full-length TRAIL and tested its efficacy in several cancer cell lines. Ad-TRAIL-infected cancer cells localized full-length TRAIL protein to the cytoplasm and released same-sized TRAIL in the media. Ad-TRAIL was found to induce apoptotic cell death in several cancer cell lines resistant to soluble TRAIL (A549, SKOV3, HT-29 and LNCap) and in TRAIL-sensitive cell lines. Ad-TRAIL, but not soluble TRAIL, induced apoptotic cell death in TRAIL-resistant cell lines, manifested by an increased sub-G1 proportion, caspase-3 activation and PARP cleavage. Ad-TRAIL also induced a media-transferable bystander effect, but only in soluble TRAIL-sensitive cell lines. In conclusion, two novel characteristics of ad-TRAIL were found during this study. First, that ad-TRAIL can induce apoptotic cell death in several cancer cell lines resistant to sTRAIL. Second, that ad-TRAIL induces a media-transferable bystander effect, which is expected to increase its therapeutic value by allowing TRAIL to overcome the locally acting nature and low transduction rate commonly encountered in clinical situation.",

keywords = "Adenovirus, Apoptosis, Bystander effect, TRAIL, TRAIL resistance",

author = "Seol, \{Ja Young\} and Park, \{Kyung Ho\} and Hwang, \{Chang Il\} and Park, \{Woong Yang\} and Yoo, \{Chul Gyu\} and Kim, \{Young Whan\} and Han, \{Sung Koo\} and Shim, \{Young Soo\} and Lee, \{Choon Taek\}",

year = "2003",

month = jul,

day = "1",

doi = "10.1038/sj.cgt.7700597",

language = "English",

volume = "10",

pages = "540--548",

journal = "Cancer Gene Therapy",

issn = "0929-1903",

publisher = "Springer Nature",

number = "7",

}

TY - JOUR

T1 - Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect

AU - Seol, Ja Young

AU - Park, Kyung Ho

AU - Hwang, Chang Il

AU - Park, Woong Yang

AU - Yoo, Chul Gyu

AU - Kim, Young Whan

AU - Han, Sung Koo

AU - Shim, Young Soo

AU - Lee, Choon Taek

PY - 2003/7/1

Y1 - 2003/7/1

N2 - TRAIL is a cytokine with a unique ability to induce apoptosis selectively in many transformed cell lines. The instability of TRAIL and the resistance of some cancer cells to TRAIL present the main obstacles for clinical experimentation. We generated an adenovirus expressing full-length TRAIL and tested its efficacy in several cancer cell lines. Ad-TRAIL-infected cancer cells localized full-length TRAIL protein to the cytoplasm and released same-sized TRAIL in the media. Ad-TRAIL was found to induce apoptotic cell death in several cancer cell lines resistant to soluble TRAIL (A549, SKOV3, HT-29 and LNCap) and in TRAIL-sensitive cell lines. Ad-TRAIL, but not soluble TRAIL, induced apoptotic cell death in TRAIL-resistant cell lines, manifested by an increased sub-G1 proportion, caspase-3 activation and PARP cleavage. Ad-TRAIL also induced a media-transferable bystander effect, but only in soluble TRAIL-sensitive cell lines. In conclusion, two novel characteristics of ad-TRAIL were found during this study. First, that ad-TRAIL can induce apoptotic cell death in several cancer cell lines resistant to sTRAIL. Second, that ad-TRAIL induces a media-transferable bystander effect, which is expected to increase its therapeutic value by allowing TRAIL to overcome the locally acting nature and low transduction rate commonly encountered in clinical situation.

AB - TRAIL is a cytokine with a unique ability to induce apoptosis selectively in many transformed cell lines. The instability of TRAIL and the resistance of some cancer cells to TRAIL present the main obstacles for clinical experimentation. We generated an adenovirus expressing full-length TRAIL and tested its efficacy in several cancer cell lines. Ad-TRAIL-infected cancer cells localized full-length TRAIL protein to the cytoplasm and released same-sized TRAIL in the media. Ad-TRAIL was found to induce apoptotic cell death in several cancer cell lines resistant to soluble TRAIL (A549, SKOV3, HT-29 and LNCap) and in TRAIL-sensitive cell lines. Ad-TRAIL, but not soluble TRAIL, induced apoptotic cell death in TRAIL-resistant cell lines, manifested by an increased sub-G1 proportion, caspase-3 activation and PARP cleavage. Ad-TRAIL also induced a media-transferable bystander effect, but only in soluble TRAIL-sensitive cell lines. In conclusion, two novel characteristics of ad-TRAIL were found during this study. First, that ad-TRAIL can induce apoptotic cell death in several cancer cell lines resistant to sTRAIL. Second, that ad-TRAIL induces a media-transferable bystander effect, which is expected to increase its therapeutic value by allowing TRAIL to overcome the locally acting nature and low transduction rate commonly encountered in clinical situation.

KW - Adenovirus

KW - Apoptosis

KW - Bystander effect

KW - TRAIL

KW - TRAIL resistance

UR - https://www.scopus.com/pages/publications/0038156082

U2 - 10.1038/sj.cgt.7700597

DO - 10.1038/sj.cgt.7700597

M3 - Article

C2 - 12833134

AN - SCOPUS:0038156082

SN - 0929-1903

VL - 10

SP - 540

EP - 548

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

IS - 7

ER -

Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this