A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Eun Young Song; Kyoung A. Kim; Yung Dai Kim; Dur Han Kwon; Hong Soo Lee; Cheorl Ho Kim; Tae Wha Chung; Si Myung Byun

doi:10.1023/A:1005641115696

A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Eun Young Song
, Kyoung A. Kim
, Yung Dai Kim
, Dur Han Kwon
, Hong Soo Lee
, Cheorl Ho Kim
, Tae Wha Chung
, Si Myung Byun

Korea Research Institute of Bioscience and Biotechnology
Dongguk University
Korea Advanced Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Biotechnology Letters
Volume	22
Issue number	3
DOIs	https://doi.org/10.1023/A:1005641115696
State	Published - 2000
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Hepatocellular carcinoma
Immunoaffinity column
Monoclonal antibody
N- acetylglucosaminyltransferase

Access to Document

10.1023/A:1005641115696

Cite this

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title = "A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues",

abstract = "Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.",

keywords = "Hepatocellular carcinoma, Immunoaffinity column, Monoclonal antibody, N- acetylglucosaminyltransferase",

author = "Song, \{Eun Young\} and Kim, \{Kyoung A.\} and Kim, \{Yung Dai\} and Kwon, \{Dur Han\} and Lee, \{Hong Soo\} and Kim, \{Cheorl Ho\} and Chung, \{Tae Wha\} and Byun, \{Si Myung\}",

year = "2000",

doi = "10.1023/A:1005641115696",

language = "English",

volume = "22",

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journal = "Biotechnology Letters",

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}

TY - JOUR

T1 - A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

AU - Song, Eun Young

AU - Kim, Kyoung A.

AU - Kim, Yung Dai

AU - Kwon, Dur Han

AU - Lee, Hong Soo

AU - Kim, Cheorl Ho

AU - Chung, Tae Wha

AU - Byun, Si Myung

PY - 2000

Y1 - 2000

N2 - Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

AB - Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

KW - Hepatocellular carcinoma

KW - Immunoaffinity column

KW - Monoclonal antibody

KW - N- acetylglucosaminyltransferase

UR - https://www.scopus.com/pages/publications/0034051786

U2 - 10.1023/A:1005641115696

DO - 10.1023/A:1005641115696

M3 - Article

AN - SCOPUS:0034051786

SN - 0141-5492

VL - 22

SP - 201

EP - 204

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Abstract

UN SDGs

Keywords

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