A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Eun Young Song; Kyoung A. Kim; Yung Dai Kim; Dur Han Kwon; Hong Soo Lee; Cheorl Ho Kim; Tae Wha Chung; Si Myung Byun

doi:10.1023/A:1005641115696

A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Eun Young Song, Kyoung A. Kim, Yung Dai Kim, Dur Han Kwon, Hong Soo Lee, Cheorl Ho Kim, Tae Wha Chung, Si Myung Byun

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Biotechnology Letters
Volume	22
Issue number	3
DOIs	https://doi.org/10.1023/A:1005641115696
State	Published - 2000
Externally published	Yes

Keywords

Hepatocellular carcinoma
Immunoaffinity column
Monoclonal antibody
N- acetylglucosaminyltransferase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1023/A:1005641115696

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title = "A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues",

abstract = "Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.",

keywords = "Hepatocellular carcinoma, Immunoaffinity column, Monoclonal antibody, N- acetylglucosaminyltransferase",

author = "Song, \{Eun Young\} and Kim, \{Kyoung A.\} and Kim, \{Yung Dai\} and Kwon, \{Dur Han\} and Lee, \{Hong Soo\} and Kim, \{Cheorl Ho\} and Chung, \{Tae Wha\} and Byun, \{Si Myung\}",

year = "2000",

doi = "10.1023/A:1005641115696",

language = "English",

volume = "22",

pages = "201--204",

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TY - JOUR

T1 - A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

AU - Song, Eun Young

AU - Kim, Kyoung A.

AU - Kim, Yung Dai

AU - Kwon, Dur Han

AU - Lee, Hong Soo

AU - Kim, Cheorl Ho

AU - Chung, Tae Wha

AU - Byun, Si Myung

PY - 2000

Y1 - 2000

N2 - Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

AB - Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

KW - Hepatocellular carcinoma

KW - Immunoaffinity column

KW - Monoclonal antibody

KW - N- acetylglucosaminyltransferase

UR - https://www.scopus.com/pages/publications/0034051786

U2 - 10.1023/A:1005641115696

DO - 10.1023/A:1005641115696

M3 - Article

AN - SCOPUS:0034051786

SN - 0141-5492

VL - 22

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Abstract

Keywords

UN SDGs

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