A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

Myeong Seon Jeong; Eunha Kim; Hyo Jin Kang; Eun Joung Choi; Alvin R. Cho; Sang J. Chung; Seung Bum Park

doi:10.1039/c2cc31377d

A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

Myeong Seon Jeong
, Eunha Kim
, Hyo Jin Kang
, Eun Joung Choi
, Alvin R. Cho
, Sang J. Chung
, Seung Bum Park

Korea Research Institute of Bioscience and Biotechnology
Seoul National University
University of Washington

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

Original language	English
Pages (from-to)	6553-6555
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	52
DOIs	https://doi.org/10.1039/c2cc31377d
State	Published - 6 Jun 2012
Externally published	Yes

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title = "A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase{\textemdash}a dual-specific protein tyrosine phosphatase",

abstract = "We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).",

author = "Jeong, \{Myeong Seon\} and Eunha Kim and Kang, \{Hyo Jin\} and Choi, \{Eun Joung\} and Cho, \{Alvin R.\} and Chung, \{Sang J.\} and Park, \{Seung Bum\}",

year = "2012",

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day = "6",

doi = "10.1039/c2cc31377d",

language = "English",

volume = "48",

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journal = "Chemical Communications",

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T1 - A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

AU - Jeong, Myeong Seon

AU - Kim, Eunha

AU - Kang, Hyo Jin

AU - Choi, Eun Joung

AU - Cho, Alvin R.

AU - Chung, Sang J.

AU - Park, Seung Bum

PY - 2012/6/6

Y1 - 2012/6/6

N2 - We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

AB - We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

UR - https://www.scopus.com/pages/publications/84862164172

U2 - 10.1039/c2cc31377d

DO - 10.1039/c2cc31377d

M3 - Article

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AN - SCOPUS:84862164172

SN - 1359-7345

VL - 48

SP - 6553

EP - 6555

JO - Chemical Communications

JF - Chemical Communications

IS - 52

ER -

A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

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