A PDMS based integrated PCR microchip for genetic analysis

Sandeep Kumar Jha; You Cheol Jang; Rohit Chand; Kamrul Islam; Yong Sang Kim

A PDMS based integrated PCR microchip for genetic analysis

Sandeep Kumar Jha
, You Cheol Jang
, Rohit Chand
, Kamrul Islam
, Yong Sang Kim

Myongji University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The 193 bp PCR amplicon obtained through on-chip PCR was confirmed in case of MCF 10a cells through agarose gel electrophoresis, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for entire reaction was less than 90 min. Therefore, we propose that such microchip can be helpful in predicting the risk of cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.

Original language	English
Title of host publication	BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices
Pages	273-275
Number of pages	3
State	Published - 2011
Externally published	Yes
Event	International Conference on Biomedical Electronics and Devices, BIODEVICES 2011 - Rome, Italy Duration: 26 Jan 2011 → 29 Jan 2011

Publication series

Name	BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices

Conference

Conference	International Conference on Biomedical Electronics and Devices, BIODEVICES 2011
Country/Territory	Italy
City	Rome
Period	26/01/11 → 29/01/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

ITO microheater
Lab-on-a-chip
PCR microchip
PDMS
SMAD4

Cite this

@inproceedings{858371f0c7b34ddf99c0168585aa8f6f,

title = "A PDMS based integrated PCR microchip for genetic analysis",

abstract = "An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The 193 bp PCR amplicon obtained through on-chip PCR was confirmed in case of MCF 10a cells through agarose gel electrophoresis, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for entire reaction was less than 90 min. Therefore, we propose that such microchip can be helpful in predicting the risk of cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.",

keywords = "ITO microheater, Lab-on-a-chip, PCR microchip, PDMS, SMAD4",

author = "Jha, \{Sandeep Kumar\} and Jang, \{You Cheol\} and Rohit Chand and Kamrul Islam and Kim, \{Yong Sang\}",

year = "2011",

language = "English",

isbn = "9789898425379",

series = "BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices",

pages = "273--275",

booktitle = "BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices",

note = "International Conference on Biomedical Electronics and Devices, BIODEVICES 2011 ; Conference date: 26-01-2011 Through 29-01-2011",

}

Jha, SK, Jang, YC, Chand, R, Islam, K & Kim, YS 2011, A PDMS based integrated PCR microchip for genetic analysis. in BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices. BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices, pp. 273-275, International Conference on Biomedical Electronics and Devices, BIODEVICES 2011, Rome, Italy, 26/01/11.

A PDMS based integrated PCR microchip for genetic analysis. / Jha, Sandeep Kumar; Jang, You Cheol; Chand, Rohit et al.
BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices. 2011. p. 273-275 (BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A PDMS based integrated PCR microchip for genetic analysis

AU - Jha, Sandeep Kumar

AU - Jang, You Cheol

AU - Chand, Rohit

AU - Islam, Kamrul

AU - Kim, Yong Sang

PY - 2011

Y1 - 2011

N2 - An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The 193 bp PCR amplicon obtained through on-chip PCR was confirmed in case of MCF 10a cells through agarose gel electrophoresis, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for entire reaction was less than 90 min. Therefore, we propose that such microchip can be helpful in predicting the risk of cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.

AB - An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The 193 bp PCR amplicon obtained through on-chip PCR was confirmed in case of MCF 10a cells through agarose gel electrophoresis, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for entire reaction was less than 90 min. Therefore, we propose that such microchip can be helpful in predicting the risk of cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.

KW - ITO microheater

KW - Lab-on-a-chip

KW - PCR microchip

KW - PDMS

KW - SMAD4

UR - https://www.scopus.com/pages/publications/79960186547

M3 - Conference contribution

AN - SCOPUS:79960186547

SN - 9789898425379

T3 - BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices

SP - 273

EP - 275

BT - BIODEVICES 2011 - Proceedings of the International Conference on Biomedical Electronics and Devices

T2 - International Conference on Biomedical Electronics and Devices, BIODEVICES 2011

Y2 - 26 January 2011 through 29 January 2011

ER -

A PDMS based integrated PCR microchip for genetic analysis

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this