A new double-stranded RNA mycovirus from Pleurotus ostreatus (ASI 2504)

Jin Kyung Lee; Kang Hyo Lee; Hyekyung Shim; Joo Sung Yang; Gyu Hyun Kim; Won Sik Kong; Young Bok Yoo; Daehyun Kim; Donggiun Kim; Sukchan Lee

doi:10.5423/PPJ.2006.22.1.068

A new double-stranded RNA mycovirus from Pleurotus ostreatus (ASI 2504)

Jin Kyung Lee
, Kang Hyo Lee
, Hyekyung Shim
, Joo Sung Yang
, Gyu Hyun Kim
, Won Sik Kong
, Young Bok Yoo
, Daehyun Kim
, Donggiun Kim
, Sukchan Lee

Department of Integrative Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A new virus with a dsRNA genome was isolated and characterized from the Suhan-neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.

Original language	English
Pages (from-to)	68-74
Number of pages	7
Journal	Plant Pathology Journal
Volume	22
Issue number	1
DOIs	https://doi.org/10.5423/PPJ.2006.22.1.068
State	Published - 2006

Keywords

Pleurotus ostreatus
Virus
dsRNA

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10.5423/PPJ.2006.22.1.068

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title = "A new double-stranded RNA mycovirus from Pleurotus ostreatus (ASI 2504)",

abstract = "A new virus with a dsRNA genome was isolated and characterized from the Suhan-neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.",

keywords = "Pleurotus ostreatus, Virus, dsRNA",

author = "Lee, \{Jin Kyung\} and Lee, \{Kang Hyo\} and Hyekyung Shim and Yang, \{Joo Sung\} and Kim, \{Gyu Hyun\} and Kong, \{Won Sik\} and Yoo, \{Young Bok\} and Daehyun Kim and Donggiun Kim and Sukchan Lee",

year = "2006",

doi = "10.5423/PPJ.2006.22.1.068",

language = "English",

volume = "22",

pages = "68--74",

journal = "Plant Pathology Journal",

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publisher = "Korean Society of Plant Pathology",

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TY - JOUR

T1 - A new double-stranded RNA mycovirus from Pleurotus ostreatus (ASI 2504)

AU - Lee, Jin Kyung

AU - Lee, Kang Hyo

AU - Shim, Hyekyung

AU - Yang, Joo Sung

AU - Kim, Gyu Hyun

AU - Kong, Won Sik

AU - Yoo, Young Bok

AU - Kim, Daehyun

AU - Kim, Donggiun

AU - Lee, Sukchan

PY - 2006

Y1 - 2006

N2 - A new virus with a dsRNA genome was isolated and characterized from the Suhan-neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.

AB - A new virus with a dsRNA genome was isolated and characterized from the Suhan-neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.

KW - Pleurotus ostreatus

KW - Virus

KW - dsRNA

UR - https://www.scopus.com/pages/publications/84885577068

U2 - 10.5423/PPJ.2006.22.1.068

DO - 10.5423/PPJ.2006.22.1.068

M3 - Article

AN - SCOPUS:84885577068

SN - 1598-2254

VL - 22

SP - 68

EP - 74

JO - Plant Pathology Journal

JF - Plant Pathology Journal

IS - 1

ER -

A new double-stranded RNA mycovirus from Pleurotus ostreatus (ASI 2504)

Abstract

Keywords

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