A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

Jayeon Song; Vo Minh Hoa; Jungmin Yoo; Sanghoon Oh; Hyeryeon Im; Daeho Park; Gwangrog Lee

doi:10.1039/c7ra00388a

A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

Jayeon Song
, Vo Minh Hoa
, Jungmin Yoo
, Sanghoon Oh
, Hyeryeon Im
, Daeho Park
, Gwangrog Lee

Gwangju Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.

Original language	English
Pages (from-to)	14917-14922
Number of pages	6
Journal	RSC Advances
Volume	7
Issue number	24
DOIs	https://doi.org/10.1039/c7ra00388a
State	Published - 2017
Externally published	Yes

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10.1039/c7ra00388a

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title = "A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities",

abstract = "Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.",

author = "Jayeon Song and Hoa, \{Vo Minh\} and Jungmin Yoo and Sanghoon Oh and Hyeryeon Im and Daeho Park and Gwangrog Lee",

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T1 - A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

AU - Song, Jayeon

AU - Hoa, Vo Minh

AU - Yoo, Jungmin

AU - Oh, Sanghoon

AU - Im, Hyeryeon

AU - Park, Daeho

AU - Lee, Gwangrog

PY - 2017

Y1 - 2017

N2 - Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.

AB - Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.

UR - https://www.scopus.com/pages/publications/85014816800

U2 - 10.1039/c7ra00388a

DO - 10.1039/c7ra00388a

M3 - Article

AN - SCOPUS:85014816800

SN - 2046-2069

VL - 7

SP - 14917

EP - 14922

JO - RSC Advances

JF - RSC Advances

IS - 24

ER -

A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

Abstract

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